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PnLTA induces NO and TNF-α production. A and B, Cultures of the murine macrophage cell line RAW 264.7 were stimulated with PnLTA for the indicated time periods. The culture supernatants were analyzed for (A) nitrite levels or for (B) TNF-α levels. All error bars in the figures indicate SD. C, The cells were also stimulated with PnLTA, StLTA, or E. coli LPS at the indicated concentrations for 48 h, and the supernatants were analyzed for NO2 levels.
