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. Author manuscript; available in PMC: 2007 Jan 1.
Published in final edited form as: J Immunol. 2006 Jan 1;176(1):573–579. doi: 10.4049/jimmunol.176.1.573

FIGURE 2.

FIGURE 2.

A, LTA induces mRNA synthesis for iNOS and TNF-α but not for IFN-β. RAW 264.7 cells were stimulated with PnLTA, StLTA, or E. coli LPS for 3 h at the indicated concentrations. RT-PCR products of IFN-β, iNOS, TNF-α, and β-actin mRNA were separated in an agarose gel and visualized by ethidium bromide staining. B, NO production by the cells stimulated with PnLTA can be suppressed with a NO synthase inhibitor (l-NAME, ■) but not with an inert control molecule (d-NAME, □). ***, Significant reduction (p < 0.001) in nitrite levels by inhibitors. C, NO production by the cells stimulated with PnLTA can be suppressed with 2-IPD (iminopiperidine), an iNOS-specific inhibitor.