FIGURE 4.
A synthetic lipopeptide, Pam3CSK4, induces NO production, and NO production requires PAFR signaling. A, RAW 264.7 cells were stimulated with Pam3CSK4 at the indicated concentrations for 16 or 48 h for assessment of TNF-α production (○) or of NO production (●), respectively. B and C, The cells were pretreated with varying concentrations of CV6209 or ABT-491 for 1 h and then stimulated with the Pam3CSK4 for 16 or 48 h for assessment of TNF-α production (lines) or of NO production (bars), respectively. ***, Significant (p < 0.001) reduction in NO production by an inhibitor.