FIG. 4.

Subcellular localization of F and M proteins in cells infected with rSV5 harboring F protein cytoplasmic tail truncations. CV-1 cells on glass coverslips were infected with the indicated viruses. At 16 h p.i., cells were fixed with formaldehyde (and for M protein staining, the cells were permeabilized with 0.1% saponin) and bound with MAbs specific for SV5 F (Fla) or M (M-h) and then with FITC-conjugated goat anti-mouse secondary antibody. Fluorescence was examined with a Zeiss LSM 410 confocal microscope.