FIG. 5.

Polypeptide composition of rSV5 harboring F protein cytoplasmic tail truncations. rSV5 and representative rSV5 F cytoplasmic tail-truncated viruses were grown in MDBK cells. For total viral polypeptide composition, cells were metabolically labeled with [³⁵S]-Promix. Virions were purified through a 35% sucrose cushion. (A) Radiolabeled virions were fractionated by SDS-PAGE, and polypeptide radioactivity was quantified on a Fuji BioImager 1000. Incorporation of F protein into virions was calculated by quantification of the F₁ band. The histogram indicates the average data from three independent experiments. (B) Nonradiolabeled virions were fractionated by SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed with antibodies specific for F₁, F₂, and M, followed by the addition of alkaline phosphatase-conjugated secondary antibodies. Chemiluminescence was imaged on a STORM 860 system.