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. 2006 Feb 17;2(2):e10. doi: 10.1371/journal.ppat.0020010

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The assay was done as previously described [1]. Normal dNTPs were present in the reaction at 10.0 μM each. Increasing concentrations of AZTTP were added, and the level of radioactive [α−³²P]dCTP incorporated into the template/primer was measured. The level of radioactivity incorporated in the absence of analog was considered 100% activity; the other reactions were normalized to this value. The error bars are included; however, they are partially obscured by the data symbols. RT1 and RT2 designate the HIV RT backbone in which the Q151M mutation was made: HIV-1 or HIV-2.