FIG. 2.

Overexpression of PML results in the formation of large nuclear structures (ND10) containing CBP and Daxx. pHF fibroblast slide cultures were infected with Bac-PML (d to f and j to l) or wild-type baculovirus (a to c and g to i) as described in Materials and Methods. The cells were fixed and reacted with a mouse monoclonal antibody to PML (b, c, e, f, h, i, k, and l) or a rabbit polyclonal antibody to CBP (a, c, d, and f) or Daxx (g, i, j, and l). The secondary antibodies were anti-mouse immunoglobulin G (IgG) conjugated to fluorescein isothiocyanate and an anti-rabbit IgG conjugated to Texas red. Left and middle columns, single-color images captured separately; right column, merged images. The yellow visualized in the overlaid images represents colocalization of PML and CBP or PML and Daxx. The images were captured with a Zeiss camera (AxioCam) and software provided by Zeiss. The digitized images were not modified subsequent to capture.