FIG. 4.

Flow cytometric analysis of lymphocytes from L(EV)-immunized mice. Peripheral blood lymphocytes (Experiment 1) or splenic lymphocytes (Experiment 2) from naïve mice or anti-CD4-treated mice or anti-CD8-treated mice and then immunized with L(EV) were stained with fluorescein isothiocyanate-labeled anti-CD3, anti-CD4, and anti-CD8. Nonspecific labeling (shaded area, experiment 1) was determined by using an isotype-matched, fluorescein isothiocyanate-labeled antibody. Cells were analyzed on a FACSCalibur instrument using CellQuest software. The percentages of positive stained cells are shown (Experiment 2). Peripheral blood lymphocytes or spleens were processed individually from three mice/group. The data shown are from an individual mouse from each group.