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. Author manuscript; available in PMC: 2006 Feb 14.
Published in final edited form as: Nature. 2005 Aug 11;436(7052):845–847. doi: 10.1038/nature03864

Figure 2.

Figure 2

The hangAE10 mutation disrupts a gene encoding a novel zinc-finger protein. a. Genomic map of the hang locus (CG32575). The 8 exons are shown as boxes; gray shading indicates protein-coding regions. The position of the P-element insertion AE-10 is shown.

b. Conceptual translation predicts a protein of 1901 amino acids (http://www.fruitfly.org/). The positions of an EF hand (box) and zinc-finger domains (ovals) are indicated; the U1-like zinc-fingers are marked with asterisks.

c. HANG protein is expressed broadly in the nervous system (left panel) and localized to nuclei of neurons (see also Suppl. Fig. 2). Expression is undetectable in the hangAE10 mutant (right panel).

d. Northern blot analysis shows that expression of the full length hang transcript (~7Kb in length, upper arrow) is absent (or severely reduced) in the hangAE10 mutant compared to the PZ(+) control strain. The lower panel (tub) shows expression of Tubulin 84B, used as a control.