Table 1.
Grain quality and yield traits in NILs and transgenic plants
| Line | n | AC (%) |
Starch content (%) |
GC (mm) |
Glu (mg/g) |
Pro (mg/g) | Glo (mg/g) | Alb (mg/g) | Protein content (mg/g) | Taste value |
|---|---|---|---|---|---|---|---|---|---|---|
| NIL(KY131) | 20 | 16.0 ± 0.2 | 82.5 ± 1.9 | 126.0 ± 0.8 | 45.0 ± 3.2 | 2.1 ± 0.2 | 3.4 ± 0.3 | 3.4 ± 0.3 | 75.9 ± 3.7 | 75.9 ± 1.5 |
| NIL(DHX2) | 20 | 17.1 ± 0.3 | 87.4 ± 1.8 | 130.0 ± 1.6 | 39.0 ± 3.0 | 2.4 ± 0.3 | 3.4 ± 0.2 | 3.2 ± 0.2 | 62.8 ± 3.0 | 80.0 ± 1.2 |
| P value | 7.6 × 10-4 | 2.8 × 10-2 | 3.6 × 10-2 | 2.1 × 10-5 | 2.7 × 10-1 | 8.9 × 10-1 | 4.7 × 10-2 | 5.4 × 10-5 | 1.7 × 10-8 | |
| CO- | 20 | 17.8 ± 0.6 | 82.0 ± 1.1 | 97.4 ± 7.4 | 58.6 ± 5.3 | 5.5 ± 0.5 | 2.6 ± 0.4 | 2.9 ± 0.6 | 70.6 ± 2.3 | 77.0 ± 1.0 |
| CO+ | 20 | 19.5 ± 0.4 | 86.5 ± 1.7 | 103.2 ± 8.9 | 39.7 ± 7.5 | 5.4 ± 0.4 | 2.1 ± 0.3 | 3.0 ± 0.5 | 61.7 ± 3.5 | 83.0 ± 1.3 |
| P value | 1.0 × 10-4 | 9.1 × 10-7 | 2.5 × 10-5 | 1.4 × 10-8 | 8.3 × 10-1 | 2.8 × 10-4 | 3.8 × 10-1 | 1.4 × 10-8 | 3.0 × 10-13 | |
| NIP | 20 | 20.3 ± 0.4 | 87.1 ± 1.1 | 112.3 ± 4.5 | 48.2 ± 3.7 | 3.1 ± 0.4 | 5.5 ± 0.4 | 2.7 ± 0.3 | 98.6 ± 3.7 | 87.0 ± 0.1 |
| Ghd7-KO | 20 | 15.94 ± 0.6 | 84.0 ± 0.8 | 95.0 ± 0.8 | 61.4 ± 7.9 | 3.2 ± 0.2 | 4.6 ± 0.6 | 2.7 ± 0.2 | 115.9 ± 7.7 | 84.7 ± 0.5 |
| P value | 3.2 × 10-13 | 2.1 × 10-5 | 5.8 × 10-3 | 2.6 × 10-4 | 6.3 ×; 10-1 | 1.9 × 10-3 | 7.8 × 10-1 | 4.4 × 10-4 | 2.2 × 10-3 | |
| ZH11 | 20 | 19.7 ± 0.6 | 72.6 ± 1.5 | 84.5 ± 2.4 | 52.9 ± 7.8 | 2.7 ± 0.6 | 5.3 ± 0.2 | 4.3 ± 0.4 | 107.8 ± 3.0 | 82.6 ± 1.1 |
| Ghd7-OX | 20 | 22.4 ± 0.6 | 76.7 ± 2.1 | 92.4 ± 2.2 | 42.9 ± 6.5 | 2.1 ± 0.6 | 4.9 ± 0.6 | 2.9 ± 0.4 | 103.9 ± 2.7 | 85.9 ± 0.8 |
| P value | 7.9 × 10-5 | 3.8 × 10-4 | 9.3 × 10-7 | 8.1 × 10-5 | 4.7 × 10-1 | 6.3 × 10-1 | 2.2 × 10-2 | 5.3 × 10-7 | 1.8;×10-7 |
NIL, near-isogenic line; n, number of plants with the same genotype; each replicate corresponds to an individual rice plant The data are based on field trials using randomized block designs with three replications. All data are means ± s.e.m. “+” and “-” represent transgene-positive and negative plants, respectively. CO, complemented plants. KO, knockout plants. OX, overexpression plants. AC, amylose content; GC, gel consistency; Glu, grain glutelin content; Pro, grain prolamin content; Glo, grain globulin content; Alb, grain albumin content; Taste value, a comprehensive assessment of the viscosity, hardness, and appearance of cooked rice. P values were generated by two-tailed t tests. Source data are provided as a Source Data file.