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. 2002 Sep;76(18):9271–9283. doi: 10.1128/JVI.76.18.9271-9283.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 7. — Immunofluorescence analysis of gB-2 endocytosis mutants in transiently transfected COS7 cells. For cell surface staining (E to H), cells were fixed with paraformaldehyde; for intracellular staining (A to D), they were fixed with paraformaldehyde and then permeabilized with Triton X-100. Cells were stained with polyclonal antibody R91, followed by a goat anti-rabbit IgG-FITC conjugate. Magnification, ×400. Panels: A and E, wild-type gB-2; B and F, Y827A; C and G, LL849/850AA; D and H, Y867A.