FIG. 4.

Hammer blot analysis for local infection with CaMV in inoculated leaves. Turnip leaves inoculated with pCa122 (wild type [Wt]), pCafs3 (f3), or pCafs1 (f1) were harvested 5 (A), 8 (B), and 10 (C) dpi and analyzed by hammer blotting. Local infection with CaMV was detected as brown spots, which grew larger over time. The brown spots in small squares represent signals from 300 ng (in 3 μl) of purified CaMV. The faint bluish background is an artifact caused by the image scanner used, but was left uncorrected because it highlights background brown staining that represents leaf images.