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. 2002 Oct;76(19):9934–9951. doi: 10.1128/JVI.76.19.9934-9951.2002

FIG. 6.

FIG. 6.

Immunogold labeling of HSV-1-infected neurons for tegument proteins and Golgi markers. (A) Intracytoplasmic enveloped virions and vesicles double labeled with giantin, indicative of cis-Golgi (10-nm gold particles [small arrows]) and VP13/14 (silver-enhanced [SE] gold particles [arrowheads]). Bar, 200 nm. (B) Cytoplasmic vesicles double labeled with mannosidase II, a finding indicative of Golgi (10-nm gold particles [arrows]) and VP16 (SE gold particles [arrowheads]). Bar, 200 nm. (C) Enveloped virion in the cytoplasm labeled with mannosidase II. Bar, 200 nm. (D) Cytoplasmic vesicle doubled labeled with TGN38, indicative of TGN (10-nm gold particles [arrow]) and VP13/14 (SE gold particles [arrowhead]). Bar, 100 nm. (E) Enveloped virion in the cytoplasm labeled with TGN38. Bar, 100 nm. (F) Unenveloped nucleocapsid next to a cytoplasmic vesicle labeled with giantin. Bar, 100 nm.