TABLE 1.
Time course quantitative TIEM analysis of VP13/14 immunolabeling on intranuclear, cytoplasmic, and extracellular HSV-1 capsids at various times postinfection
| Time (hpi) | Label intensity | % of capsids (n)a in:
|
|||
|---|---|---|---|---|---|
| Nucleus | Cytoplasm (unenveloped) | Cytoplasm (enveloped) | Extra- cellular region | ||
| 12 | Total | 100 (195) | 100 (31) | 100 (58) | 100 (36) |
| Negative | 91.5 | — | — | — | |
| Weak | 5.6 | 32.2 | — | — | |
| Moderate | — | 35.5 | 3.5 | — | |
| Dense | — | 32.2 | 96.5 | 100 | |
| 17 | Total | 100 (493) | 100 (39) | 100 (112) | 100 (76) |
| Negative | 96.8 | 48.8 | 6.3 | 13.2 | |
| Weak | 3.2 | 20.5 | 10.7 | — | |
| Moderate | — | 17.9 | 13.4 | 14.5 | |
| Dense | — | 12.8 | 69.6 | 72.3 | |
| 24 | Total | 100 (344) | 100 (36) | 100 (61) | 100 (56) |
| Negative | 88.7 | 33.3 | — | — | |
| Weak | 11.3 | 27.8 | — | 19.6 | |
| Moderate | — | 38.9 | 4.9 | — | |
| Dense | — | 95.1 | 80.4 | ||
| 24 (plus BFA) | Total | 100 (313) | 100 (37) | 100 (63) | 100 (0) |
| Negative | 93.0 | 48.7 | 22.2 | — | |
| Weak | 7.0 | 51.3 | 17.5 | — | |
| Moderate | — | — | — | — | |
| Dense | — | — | 60.3 | — | |
a
The percentage values shown were derived by counting viral particles in 15 cell profiles for each tegument protein and for each time point. —, Complete absence. n = total number of viral particles.