TABLE 2.
Time course quantitative TIEM analysis of VP16 immunolabeling on intranuclear, cytoplasmic, and extracellular HSV-1 capsids at various times postinfection
| Time (hpi) | Label intensity | % of capsids (n)a in:
|
|||
|---|---|---|---|---|---|
| Nucleus | Cytoplasm (unenveloped) | Cytoplasm (enveloped) | Extra- cellular region | ||
| 12 | Total | 100 (212) | 100 (34) | 100 (61) | 100 (58) |
| Negative | 63.7 | 5.9 | — | — | |
| Weak | 26.9 | 29.4 | — | — | |
| Moderate | 8.5 | 23.5 | — | 3.5 | |
| Dense | 0.9 | 41.2 | 100 | 96.5 | |
| 17 | Total | 100 (480) | 100 (68) | 100 (123) | 100 (103) |
| Negative | 84.8 | 44.1 | — | — | |
| Weak | 13.3 | 39.7 | 7.3 | 5.8 | |
| Moderate | 1.9 | 11.8 | 14.7 | 6.8 | |
| Dense | — | 4.4 | 78.0 | 87.4 | |
| 24 | Total | 100 (470) | 100 (123) | 100 (76) | 100 (129) |
| Negative | 54.7 | 1.4 | — | — | |
| Weak | 24.5 | 26.0 | 4.0 | 2.3 | |
| Moderate | 12.8 | 23.6 | — | — | |
| Dense | 8.0 | 48.8 | 96.0 | 97.7 | |
| 24 (plus BFA) | Total | 100 (403) | 100 (144) | 100 (84) | 100 (0) |
| Negative | 74.4 | 39.6 | 28.6 | — | |
| Weak | 20.1 | 22.2 | 5.9 | — | |
| Moderate | 5.5 | 20.8 | 13.1 | — | |
| Dense | — | 17.4 | 52.4 | — | |
a
See Table 1, footnote a.