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Representative measurement of PD in the intermediate capillary plexus. (A) Angiograms were extracted as .tif files from the OCTA scans and imported into a custom MATLAB program. (B) Images were binarized using the Otsu method, and PD was measured within the central 2-mm region (green ring). Non-perfused areas are black, and perfused areas are white. PD was calculated as the percentage of white pixels within the green ring to the total number of pixels within the green ring.
