TABLE 2.
Examination of three asymptomatic (seropositive) sheep for the presence of viral RNA in secretions and excretions over a period of 2 and 3 months
| Sheep | Month | Titer of antibody ina:
|
Presence of viral RNA inb:
|
|||||
|---|---|---|---|---|---|---|---|---|
| Plasma | Liquor | PBMC | Urine | Saliva | Nasal secretion | Lacrimal fluid | ||
| 27 | 0 | (10) | − | −/− | −/− | −/− | −/+ | |
| 1 | NDc | ND | ND | −/− | −/− | −/− | −/− | |
| 2 | (10) | − | − | −/− | −/− | +/− | −/− | |
| 3 | ND | ND | ND | −/− | +/− | −/− | −/− | |
| 12 | 0 | 20 | − | − | −/− | −/− | +/+ | −/− |
| 1 | ND | ND | ND | −/− | −/− | +/− | −/− | |
| 2 | (10) | − | − | −/− | +/− | +/− | −/− | |
| 3 | ND | ND | ND | −/− | −/− | −/− | +/− | |
| 23 | 0 | 20 | − | − | −/− | −/− | −/− | −/− |
| 1 | ND | ND | ND | −/− | −/− | −/− | −/− | |
| 2 | (10) | − | − | −/− | −/− | −/− | +/− | |
a
Antibody titers were measured by IFA using serial plasma dilutions on persistently BDV-infected MDCK cells. Titers below the defined threshold of 20 are in parentheses.
b
The detection of BDV p40 and BDV p24 RNA is given as p40/p24.
c
ND, not done.