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. 2002 Oct;76(19):9763–9772. doi: 10.1128/JVI.76.19.9763-9772.2002

FIG. 4.

FIG. 4.

pX activates the p38 MAPK pathway in 4pX-1 cells. (A) Transient-transreporter assays employing pFR-luciferase reporter (100 ng) and pFA-CHOP expression (100 ng) plasmids (Stratagene) in 3pX-1 and 4pX-1 cells ± 5 μg of tetracycline/ml. Inhibitor concentration is as follows: 10 μM SB 203580, 3 μM PP2, and 20 μM BAPTA-AM. (B) In vitro p38 immunocomplex kinase assays with WCE (150 μg) from 4pX-1 and 3pX-1 cells, isolated at the indicated times ± 5 μg of tetracycline/ml. GST-ATF2 (2 μg) substrate phosphorylation was monitored by Western blots using phospho-ATF2 antibodies. Sorbitol treatment was the positive control. Quantitation was performed by digital densitometry using the OPTIMAS 6.1 software. pX-dependent ATF2 phosphorylation was statistically significant (P < 0.03).