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. 2002 Oct;76(19):9575–9587. doi: 10.1128/JVI.76.19.9575-9587.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 1. — Vaccinia virus J1R gene encodes a late protein. (A) Alignment of deduced amino acid sequences of vaccinia virus J1R and J1R orthologues in other poxviruses. Accession numbers are from GenBank. VAC, vaccinia virus (strain WR; accession no. P07616); VAR, variola virus (India-1967/isolate IND3; accession no. NP-042122); SWP, swine poxvirus (accession no. NP-570222); SPV, sheep poxvirus (accession no. P19746); LSDV, lumpy skin disease virus (accession no. AAK85026); MYX, myxoma virus (accession no. NP-051774); SFV, Shope fibroma virus (accession no. NP-051949); YLDV, Yaba-like disease virus (accession no. NP-073450); MCV, molluscum contagiosum virus subtype 1 (accession no. NP-044026); FPV, fowl poxvirus (accession no. NP-039096). The boxed sequences are identical amino acids, and the thick lines indicate hydrophobic sequences (HB). (B) Hydropathy plot of J1R protein. a.a., amino acid. (C) Expression of J1R protein in infected cells and IMV. BSC40 cells were infected with wild-type vaccinia virus at an MOI of 5 PFU per cell and harvested at the indicated times. Lysates were separated by SDS-12% PAGE and transferred to nitrocellulose for Western blotting with rabbit antibody against J1R protein. araC, cytosine β-d-arabinofuranoside; hp.i, hours postinfection; V, purified IMV particles.

FIG. 1. — Vaccinia virus J1R gene encodes a late protein. (A) Alignment of deduced amino acid sequences of vaccinia virus J1R and J1R orthologues in other poxviruses. Accession numbers are from GenBank. VAC, vaccinia virus (strain WR; accession no. P07616); VAR, variola virus (India-1967/isolate IND3; accession no. NP-042122); SWP, swine poxvirus (accession no. NP-570222); SPV, sheep poxvirus (accession no. P19746); LSDV, lumpy skin disease virus (accession no. AAK85026); MYX, myxoma virus (accession no. NP-051774); SFV, Shope fibroma virus (accession no. NP-051949); YLDV, Yaba-like disease virus (accession no. NP-073450); MCV, molluscum contagiosum virus subtype 1 (accession no. NP-044026); FPV, fowl poxvirus (accession no. NP-039096). The boxed sequences are identical amino acids, and the thick lines indicate hydrophobic sequences (HB). (B) Hydropathy plot of J1R protein. a.a., amino acid. (C) Expression of J1R protein in infected cells and IMV. BSC40 cells were infected with wild-type vaccinia virus at an MOI of 5 PFU per cell and harvested at the indicated times. Lysates were separated by SDS-12% PAGE and transferred to nitrocellulose for Western blotting with rabbit antibody against J1R protein. araC, cytosine β-d-arabinofuranoside; hp.i, hours postinfection; V, purified IMV particles.