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Membrane and core proteins after NP-40-DTT extraction of vaccinia virus IMV. Sucrose-purified vaccinia virus IMV were incubated with buffer containing 1% NP-40 and 50 mM DTT or no DTT. After centrifugation, the supernatant (S) and the insoluble pellet (P) were analyzed with antibodies as described in Materials and Methods. c, core; m, membrane.
