FIG. 2.
Immunoblots of serum reactivity for purified L2 proteins. Immune rabbit serum at a 1:250 dilution was used to probe blots of His6-tagged, nickel-purified fusion proteins of insect cell-derived ROPV L2 (lanes 3), bacterially derived CRPV L2 (lanes 4), and HPV 16 L2 (lanes 5); included are bacterial cell lysates (lanes 1) and insect cell lysates (lanes 2). Sera from rabbits immunized with ROPV L2.1 (A), ROPV L2.2 (B), CRPV L2.1 (C), CRPV L2.2 (D), and HPV 16 L2 (E) are shown. An anti-His6 antibody was used as a control (F). Arrows, 66-kDa marker. L2 is ∼70 to 75 kDa.