FIG. 4.
RT-PCR analysis of CRPV neutralization by rabbit CRPV L2 peptide immune serum. (Top) Amplimers of the E1^E4 transcript. Lanes 1 and 15, virus only; lanes 2 and 16, uninfected cells; lanes 3 to 8 and 17 to 19, CRPV L2.2 serum diluted 1:80 (lane 3), 1:40 (lane 4), 1:20 (lanes 5 and 18), 1:10 (lanes 6 and 19), and 1:5 (lane 7) and undiluted preimmune serum (lanes 8 and 17); lanes 9 to 11 and 20 and 21, CRPV L2.1 serum diluted 1:20 (lanes 9 and 21) and 1:10 (lane 10) and undiluted preimmune serum (lanes 11 and 20); lane 12, 1:5 dilution of ROPV L2.1 serum; lane 13, ROPV L2.2 serum; lane 14, HPV 16 serum. (Bottom) β actin controls for each lane.