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. 2002 Oct;76(19):9787–9797. doi: 10.1128/JVI.76.19.9787-9797.2002

TABLE 2.

Transmission of infection by injected cell-free or cell-associated wild-type and nef-defective SIVmac239

Animal Inoculuma Plasma p27 Agb Anti-SIV Abb Time of death p.i.c
G544 3 × 105 wt-DCs + + 13 mo
AR 82 3 × 105 wt-DCs + − 19 wk
H917 3 × 105 wt-DCs + + 8 mo
H522 1.2 × 106 wt-DCs + NDe 3 mo
I686d 9 × 105 wt-DCs + ND 3 mo
M846 3 × 105 delta-DCs + + 17 mo
K080 3 × 105 delta-DCs + + 27 mo
P741 3 × 105 delta-DCs + + 37 mo
P318 3 × 105 delta-DCs + + 28 mo
AT 53 1.2 × 106 delta-DCs + ND 3 mo
C577 1.2 × 106 delta-DCs + ND 3 mo
L721 3 × 105 wt-T cells + + 15 mo
L702 3 × 105 wt-T cells + + 6 mo
J696 1.2 × 106 wt-T cells + ND 3 mo
J995 1.2 × 106 wt-T cells + ND 3 mo
M177 3 × 105 delta-T cells + − 24 mo
L507 3 × 105 delta-T cells + + Alive 3 yr
AR 73 1 × 105 delta, s.c. + − 3 mo
N992 1 × 102 delta, s.c. + + Alive 3 yr
M806 1 × 105 wt, s.c. + + 13 mo
AR 79 1 × 102 wt, s.c. + + 4.5 mo
AR 70 1 × 105 wt, s.c. + + 3 mo
G402 1 × 105 wt, s.c. + + 7 mo
AT 88 1 × 102 wt, i.v. ND − 11 wk
AR 80 1 × 102 wt, i.v. ND + 19 mo
AT 62 2.35 × 104 delta, i.v. ND + Alive 3.5 yr
AT 84 2.35 × 104 delta, i.v. ND + 19 mo
AR 83 2.35 × 104 delta, i.v. ND + Alive 3.5 yr
AR 85 2.35 × 104 delta, i.v. ND + 13 mo
a

wt-DCs, wild-type virus-infected DCs; delta-DCs; delta nef virus-infected DCs.

b

Plasma p27 Ag was detected within 1 to 3 weeks of infection, and anti-SIV envelope Abs we detected by weeks 8 to 12 p.i., varying as expected between donors. Positivity was confirmed in at least one follow-up measurement, and negative results reflect animals that never seroconverted during that testing period.

c

Animals were euthanized if they appeared to be ailing, and necropsy samples were analyzed where possible. H522, I686, AT 53, C577, J696, and J995 were euthanized and necropsied after 3 months.

d

I686 received only 3 × 105 cells at three sites. Note that the i.v. delta nef virus-infected animals AT 62, AT 84, AR 83, and AR85 were i.v. challenged with 100 TCID50 of wild-type virus 15 weeks after their initial infection with delta nef virus for use in other ongoing immune studies. In other continuing studies, animals M846, K080, P318, P741, M177, L507, and N992 were similarly i.v. challenged with wild-type virus approximately 11 months after injection of cell-associated or cell-free delta nef virus. All times of death are relative to the initial infection with delta nef virus.

e

ND, not done.