TABLE 4.

Increased numbers of CD169-positive, SIV RNA-expressing cells in LNs 14 days after s.c. reinjection of cell-associated virus

Animal	Inoculum (s.c.)c	No. of RNA-positive cells pera:		% of RNA-positive cells expressing CD169b
		Section	mm2
I686	wt-DCs	890	24.18	49.36
H522	wt-DCs	853	29	25.61
C577	delta-DCs	135	10.5	NDd
AT 53	delta-DCs	20	0.9	ND
J696	wt-T cells	1,495	48.14	28.78
J995	wt-T cells	1,758	29.42	50.52
AR 70	105 TCID50 wt	185	6.16	9.5
G402	105 TCID50 wt	147	16.4	1.5

^aLNs were biopsied at days 1, 3, 7, and 14 after injection of cell-associated virus and 14 days after infection with cell-free virus. As with the i.v. infected animals, few SIV-positive cells were detected before day 14 (i.e., at day 7 the numbers of SIV-positive cells ranged from 0.0 to 2.4 per mm² or 0 to 36 per section).

^bCD169 stains were performed on day 14 samples where sufficient RNA-positive profiles were available to phenotype.

^cAnimals were s.c. injected with DC- or T-cell-associated wild-type (wt) or delta nef (delta) virus or with 10⁵ TCID₅₀ of cell-free wild-type virus.

^dND, not done, as there were too few RNA-positive cells.