TABLE 3.

S. pombe strains used in this study

Name	Strain	Genotype	Description	G2 arresta
Wild type	PR109	leu1-32 ura4-D18 h−	Normal cell cycle	+
cdc2-1w	JM305	cdc2-1w leu1-32 ura4-D18 h+	Constitutively active Cdc2, insensitive to Y15 phosphorylation by Wee1	+
cdc2-3w	JM125	cdc2-3w leu1-32 ura4-D18 h−	Cdc2 active independently of Cdc25	+
cdc2-3w Δcdc25	JM192	cdc2-3w cdc25::ura4 leu1-32 ura4-D18 h	As for cdc2-3w plus no expression of Cdc25	+
wee1-50	JM604	wee1-50 leu1-32 ura4D-18 h−	Temperature-sensitive mutant of Wee1	+
wee1-50 Δmik1	PR337	wee1-50 mik1::ura4 leu1.32 ura4-D18 h−	Same as for wee1-50 plus no expression of Mik1	+
wee1-50 cdc25-22	JM1657	wee1-50 cdc25-22 leu1-32 ura4-D18 h−	Same as for wee1-50 plus temperature-sensitive mutant of Cdc25	+
Δrad24	JM1172	rad24::ura4 leu1-32 ura4-D18 ade6-704 h−	No expression of Rad24	+
Δrad25	JM1173	rad25::ura4 leu1-32 ura4-D18 ade6-704 h−	No expression of Rad25	+
cdc10-129	JM195	cdc10-129 leu1.32 h−	Temperature-sensitive arrest in G1	+

^a+, positive for G₂ arrest.