FIG. 2.

NOC blocks nuclear targeting of Ad2-TR in A549 cells. Ad2-TR was cold bound to A549 cells not pretreated or pretreated with different concentrations of NOC. Cells were warmed for the indicated times at 37°C in the presence or in the absence of NOC and then fixed with PHEMO-fix. (A) Entire set of projected confocal laser scanning microscopy sections of representative cells depicting Ad2-TR (red), MTs (green), and the nucleus (4′,6′-diamidino-2-phenylindole; dark blue). Note the disappearance of the MTs with increasing concentrations of NOC and the lack of nuclear accumulation of Ad2-TR. Bar, 10 μm. (B) Quantification of the subcellular localization of Ad2-TR in the periphery, the cytoplasm, and the nuclear and perinuclear region of cells treated with different concentrations of NOC (n, number of cells analyzed). (C) Quantification of the subcellular localization of Ad2-TR in A549 control cells over time. Note that the nuclear transport of Ad2-TR peaks at 80 to 90 min p.i., with corresponding reductions in peripheral and cytoplasmic particles. (D) Quantification of the subcellular localization of Ad2-TR in the absence of MTs (NOC treatment). Note the lack of nuclear transport at times before 200 min p.i. and the slight increase in nuclear accumulation at 300 min p.i. Error bars indicate standard errors of the means.