FIG. 4.

NOC inhibits the nuclear targeting of HSV-1 in Vero and PtK₂ cells. (A) Vero cells were infected in the absence or in the presence of different concentrations of NOC with 50 PFU of HSV-1 per cell for 2 h and then fixed in methanol after preextraction. Samples were stained with anti-HC (green) and antitubulin (red) and analyzed by confocal laser scanning microscopy. Note the disappearance of the MTs with increasing concentrations of NOC and the lack of nuclear HSV-1 capsids. (B to D) Quantification of subcellular localization of HSV-1 in PtK₂ cells infected with 80 PFU per cell in the absence of drug (B), in the presence of 50 μM NOC (C), or in the presence of 10 μM paclitaxel (D). Cells were fixed at 30, 120, or 180 min p.i. and labeled with anti-LC. n, number of cells. Error bars indicate standard errors of the means. MTs in Vero cells were completely depolymerized at lower concentrations of NOC than were those in PtK₂ cells (data not shown).