FIG. 6.

BVDV infection and tunicamycin treatment decrease intracellular GSH levels in MDBK cells. (A) Northern blot analysis of BVDV-infected MDBK cells harvested at the indicated times postinfection and probed with a radiolabeled cDNA probe from the human GCLC gene. The Northern blots were stripped and probed with a radiolabeled RNA probe specific for the mouse GAPDH gene. 5M and 30M, 5 and 30 h post-mock infection, respectively. (B) Intracellular levels of GSH were measured in BVDV-infected MDBK cell lysates. The GSH levels were normalized to total protein content in the cell lysates. (C) GSH levels were measured in mock-infected or BVDV-infected MDBK cells harvested at 24 h postinfection (h.p.i.). In separate cultures, MDBK cells were treated with 1 μg of tunicamycin (TUN) or irradiated with 40 μJ of UV light (218 nm). GSH levels were normalized to total protein content in the cell extracts.