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Cellular localization of wt L1 and L2 proteins. (A) COS-7 cells were infected with vaccinia viruses recombinant for HPV33 L1 or HPV33 L2 and immunostained after 8 h. Colocalization of L2 with promyelocytic leukemia protein (PML) is shown. (B) COS-7 cells were coinfected with both viruses; at the indicated times after infection the cells were fixed, and the capsid proteins were visualized by immunofluorescence.
