FIG. 1.

The j4R protein is expressed late during ASFV infection. Extracts from IBRS2 cells either mock infected (Mock) or infected with the Uganda ASFV isolate for the indicated times (h) in the presence (+) or absence (−) of 50 μg of cytosine arabinoside/ml were separated by SDS-PAGE, and proteins were blotted to a Hybond C membrane. Blots were probed with anti-j4R serum (1 in 250 dilution), followed by goat anti-rabbit HRP-conjugated secondary antibody (1 in 2,000 dilution), and bound antibodies were detected by ECL. The positions of mass markers run in parallel are shown. The 35-kDa j4R protein is indicated by an arrow.