FIG. 5.

Analysis of pol cleavage products in DIBA-2-treated MMLV. MMLV produced in the presence of DIBA-2 (lanes 1 and 2) was incubated with (lanes 2) or without (lanes 1) DTT; a control sample produced without DIBA-2 (lanes 3) was also analyzed. Immunoblotting was performed with rabbit anti-PR antiserum (A); the membrane was then stripped and reprobed with DC-1, a rabbit anti-(RT-IN) antiserum (B) that was a gift of Judith G. Levin, National Institute of Child Health and Human Development. Autoradiography performed between stripping and reprobing showed that the signal from the first antibody was completely removed by stripping. (C) Inferred sequence of cleavages in Gag-Pol maturation. The figure shows the cleavage intermediates present in MMLV produced in DIBA-2 as well as the final cleavage products found in DTT-treated or control mature virions.