FIG. 6.

In situ hybridization in cells transfected with subgenomic pgTat/pRev constructs. (A) Signal for env intron probe (12-nm colloidal gold) is diffusely distributed on the RNP-containing nuclear fibrils (arrowheads) and in the cytoplasm. (B) LMB treatment gives rise to viral RNA clustering, revealed here by the env intron probe. The gold particle clusters occur in the nucleoplasm and at the nuclear pores, while no signal is detected in the cytoplasm. (C) In LMB-treated cells, viral RNA detection by the rev exon probe gives rise to both clustered and diffuse labeling patterns in the nucleoplasm and also reveals RNAs diffusely distributed in the cytoplasm (arrowheads). Thin arrows show the nuclear periphery. Cy, cytoplasm. Bars, 0.2 μm.