FIG. 2.

Relative molar RNA packaging efficiencies of procapsid, S250Q, OG-treated, and P4-null particles. Individual packaging of the s, m, and l segments is analyzed as indicated in the figure. Packaging of the l segment was enhanced by addition of a nonradioactive m segment to the reaction mixtures (6). The negative control, Δs, is a derivative of the s segment containing a deletion that destroys the packaging signal and abolishes packaging activity. Results were normalized first to the amount of protein P1 in each reaction and then against the level of s-segment packaging with procapsids, which was set to 1. S250Q*, frozen and thawed S250Q particles. For better comparison of the packaging efficiencies of the OG-treated particles, a 5× multiplied version is also included.