FIG. 2.

Effect of BAL on Ad5/β-gal gene transfer to human airway epithelium displaying GPI-CAR on its apical surface. BAL obtained from stable CF patients (A) and from normal subjects (B) (50 μl) was applied to the apical surface of human airway epithelium displaying GPI-CAR for 30 min at 37°C. The control sample received 50 μl of medium alone. To this solution, 10 MOI of Ad5/β-gal was added for 1 h at 37°C. Epithelia were then evaluated 2 days later for β-galactosidase activity. β-Galactosidase activity is expressed as means ± SEM, n = 40, for four CF patients (C01 to C04) and n = 48 for three non-CF subjects (N01 to N03). *, P < 0.01.