FIG. 5.

Effect of non-CF BAL (A) and CF BAL (B) on AAV5 gene transfer to human airway epithelium. Human airway epithelia were incubated with 50 μl of CF BAL, non-CF BAL, or vehicle alone (PBS) for 30 min at 37°C prior to the addition of 500 particles of recombinant AAV5/β-gal per cell to the apical surface. Cells were incubated with virus for 4 h at 37°C. After the incubation period, the virus suspension was removed, and the apical surface was washed twice in PBS. The cells were then incubated at 37°C for 14 days. After this time, β-galactosidase activity was measured as described above. The data are expressed as means ± SEM, n = 15, for three CF and three non-CF donors.