FIG. 5.

Formation of complexes between Pro11 and DNA fragments containing different numbers of Pro11 binding sites. (A) Lanes 1 to 4, DNA fragment orf11F6R2 (four binding sites); lanes 5 to 8, DNA fragment orf11F7R2 (three binding sites). (B) Lanes 1 to 4, fragment orf11F8R2 (two binding sites); lanes 5 to 8, fragment orf11F9R2 (one binding site). The binding reactions were carried out with 1 RU of Pro11 in standard buffer containing increasing amounts of NaCl (50, 125, 250, and 500 mM), as indicated by the triangles.