TABLE 4.

Immunodominant regions within HIV-1C

HIV-1C protein	aa		Amino acid sequencea	Frequency of CTL responses (% of samples)	Normalized cumulative CTL responsesb	Amino acid diversityc
	Start	End				%	P value	Mean difference, % (95% CI)
Gag	161	180	EKAFSPEVIPMFTALSEGAT	10.2	47.3	3.2	<0.0001	6.2 (4.8, 7.6)
	171	190	MFTALSEGATPQDLNTMLNT	32.2	190.5	2.5	<0.0001	6.9 (5.5, 8.4)
	291	310	EPFRDYVDRFFKTLRAEQAT	18.6	80.2	3.2	0.0062	1.9 (0.5, 3.2)
	301	320	FKTLRAEQATQDVKNWMTDT	17.0	87.7	5.5	<0.0001	4.0 (2.1, 5.9)
	331	350	KTILRALGPGATLEEMMTAC	15.3	43.7	7.2	0.0043	2.2 (0.7, 3.7)
Pol	271	290	FSVPLDEDFRKYTAFTIPSI	12.7	48.8	9.3	0.0064	−2.8 (−4.9, −0.8)
	421	440	WASQIYPGIKVRQLCKLLRG	10.9	44.8	9.9	0.12	−3.5 (−8.0, 1.0)
	521	540	KQLTEAVOKIAMESIVIWGK	10.9	27.3	11.2	<0.0001	−4.8 (−6.8, −2.9)
	894	911	AVFIHNFKRKGGIGGYSA	14.1	41.4	3.0	<0.0001	3.4 (2.5, 4.4)
	925	942	TKELQKQIIKIQNFRVYY	15.6	36.7	9.8	0.00025	−3.4 (−5.3, −1.6)
Vif	61	80	EARLVIKTYWGLOTGERDWH	12.7	32.8	18.4	<0.0001	−6.8 (−9.5, −4.0)
	71	90	GLQTGERDWHLGHGVSIEWR	16.4	41.1	9.4	0.016	2.2 (0.4, 3.9)
Vpr	31	50	VRHFPRPWLHSLGQYIYETY	21.4	52.6	19.4	<0.0001	−7.5 (−9.9, −5.0)
Tat	16	30	SQPKTACNKCYCKRC	10.7	25.0	31.8	<0.0001	−13.5 (−16.5, −10.4)
	36	50	VCFQTKGLGISYGRK	30.4	96.0	11.9	<0.0001	5.9 (3.9, 7.9)
Env	299	319	PNNNTRKSIRIGPGQTFYA	11.3	28.1	10.7	<0.0001	9.4 (7.6, 11.1)
	552	571	QSNLLRAIEAQQHMLQLTVW	10.7	25.2	4.9	<0.0001	15.1 (14.0, 16.2)
	702	721	LSIVNRVRQGYSPLSFQTLT	10.7	29.3	8.7	<0.0001	11.3 (9.3, 13.4)
	742	761	RDRSIRLVSGFLALAWDDLR	19.6	42.2	17.4	0.028	2.6 (0.3, 5.0)
Nef	1	16	MGGKWSKSSIVGWPAV	12.7	27.9	25.8	0.0059	−7.2 (−12.3, −2.1)
	67	81	GFPVRPQVPLRPMTY	12.7	45.1	9.2	<0.0001	9.4 (5.5, 13.3)
	72	86	PQVPLRPMTYKGAFD	16.4	43.8	16.1	0.20	2.5 (−1.3, 6.2)
	77	91	RPMTYKGAFDLSFFL	12.7	35.2	20.1	0.35	−0.5 (−4.7, 1.6)
	82	96	KGAFDLSFFLKEKGG	20.0	60.4	16.3	0.064	2.3 (−0.1, 4.7)
	97	111	LEGLIYSKKRQEILD	12.7	40.7	15.2	0.0095	3.4 (0.8, 6.0)
	112	126	LWVYHTQGYFPDWQN	16.4	38.4	11.1	<0.0001	7.5 (5.5, 9.6)
	122	136	PDWQNYTPGPGVRYP	18.2	50.6	9.0	<0.0001	9.7 (7.4, 11.9)
	127	141	YTPGPGVRYPLTFGW	14.5	56.9	8.5	<0.0001	10.1 (8.0, 12.2)

^aThe top eight immunodominant regions are in boldface.

^bCumulative CTL responses were normalized by the number of samples screened with a particular set of synthetic peptides.

^cThe amino acid diversity for a particular immunodominant region was compared with the amino acid diversity of the entire corresponding viral protein (P value and mean difference).