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Construction and in vivo tropism of NSV recombinants derived from dsNSV. (A) Schematic of dsNSV constructs. (B to H) Expression of functional GFP in infected cells of the cortex (B and C), hippocampus (D), brain stem (E), cerebellum (F), and lumbar spinal cord (G and H) 4 days after intracerebral inoculation of 4-week-old mice with 103 PFU of dsNSV-GFP (Nissl red counterstain) (magnifications, ×20 in panels B and E; ×40 in panels C, D, F, and G; and ×60 in panel H).
