FIG. 3.

Overexpression of BCL-2 family members alters survival but not paralysis caused by dsNSV infection. (A) Percentage of 4-week-old mice (at least 75 per group, in four separate experiments) that survived intracerebral inoculation with 10³ PFU of either dsNSV-GFP, dsNSV-BCL-2, or dsNSV-BAX. BCL-2 and BAX are both protective compared to GFP (P < 0.04 and P < 0.004, respectively). (B) Percentage of animals that did not develop hind-limb paralysis following infection. The risk of paralysis among dsNSV-BCL-2-infected mice compared to dsNSV-GFP-infected mice generated an OR of 0.87 (P = 0.19). The risk of paralysis among dsNSV-BAX-infected mice compared to dsNSV-GFP-infected mice generated an OR of 1.09 (P = 0.32). (C and D) Replication of recombinant dsNSV-derived viruses in the brains (C) and spinal cords (D) of infected mice. Each point represents the mean and SEM log₁₀ PFU per gram of tissue from at least three animals. None of the brain or spinal cord viral growth curves was statistically different from one another.