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Increased binding of SHIVSF162P3 envelope gp120 to DC-SIGN correlates with enhanced transmission to PBMCs in trans. A total of 2 × 105 HEK-293T cells transiently expressing human (A) or rhesus (B) DC-SIGN were incubated with equal amounts of the R7/3-162 or R7/3-162P3 viruses, extensively washed, and cocultivated with 2 × 106 phytohemagglutinin-stimulated PBMCs. p24 Gag antigen in culture supernatant was determined at 2, 5, and 8 days cocultivation. ○, R7/3-162; □, R7/3-162P3.
