FIG. 3.

Wild-type and Gly287Glu phage infections of the β-Gal host. β-Gal-expressing cells that had been infected with either wild-type or mutant Gly287Glu phage were resolved in sucrose gradients and analyzed for protein content by SDS-PAGE (top panels) and for particle content by native agarose gel electrophoresis (bottom panels). Load (L), samples of the total lysate; PC, procapsid peak position; φ, phage peak position. Dominant proteins (or the first letter thereof) are labeled to the left of each SDS gel, and the positions of the procapsid and phage particles are indicated to the left of each native agarose gel. The direction of sedimentation is indicated at the bottom. In the SDS gels, one-quarter of the bottommost fraction was loaded to reduce distortions caused by excessive CsCl present in the sample.