FIG. 3.

GFP-Eps15Δ95/295 expression does not prevent influenza virus infection. HeLa cells either were transiently transfected with either GFP or GFP-Eps15Δ95/295 or were left as untransfected controls before infection with influenza virus. Infection was monitored using monoclonal antibody H10, L16-4R5 against NP. (A) Entry assay. Cells were infected with ∼100 to 200 PFU per cell, and the virus was allowed to internalize for 60 min at 37°C. (B) Infection. Cells were infected with ∼1 to 5 PFU per cell and then incubated at 37°C for 4 h. (C) Low-MOI infection. Cells were infected with ∼0.1 PFU per cell and incubated at 37°C for 4 h. (D) FACS analysis of infection assay. Infection is monitored on the y axis, and transfection is monitored on the x axis. The percentage of cells within each quadrant is given.