FIG. 4.

Confocal microscopy of GFP-Eps15Δ95/295-expressing cells with influenza virus and transferrin (Tfn). HeLa cells were transiently transfected with GFP-Eps15Δ95/295 before high-MOI infection with influenza virus. The transferrin uptake assay was then performed; note that the cells were not acid washed after incubation. Influenza virus localization was determined by indirect immunofluorescence with an anti-NP monoclonal antibody. Images were created using Fluoview software and were transferred into Adobe Photoshop. The short arrow in panel b and the long arrow in panel c indicate transferrin and influenza vRNP signal localization, respectively, in untransfected cells.