FIG. 2.

Nuclear dots containing GFP-VP22 are visible by bright-field microscopy. (A) The infected cells used in the experiment shown in Fig. 1 were examined by both fluorescent microscopy (left panel) and bright-field microscopy (right panel). Corresponding fluorescent and phase-dense dots are indicated by arrows. (B) Vero cells were infected with parental virus s17 at a multiplicity of 10 and examined live by bright-field microscopy. The newly formed phase-dense nuclear dots are indicated by arrows in the 3 h.p.i. image. h.p.i., hours postinfection. (C) Vero cells infected as decribed for panel B were fixed 6 h after infection, and immunofluorescence analysis was carried out with anti-VP22 polyclonal antibody AGV30.