FIG. 7.

Relative localizations of tegument protein-containing dots with other infected cell nuclear domains. (A) Vero cells were infected with virus 183v expressing CFP-VP22 and VP26-YFP at a multiplicity of 10. At 8 h after infection, the cells were examined live for the presence of CFP-VP22 (shown in green) and VP26-YFP (shown in red). (B) Vero cells were infected with virus 166v expressing GFP-VP22 at a multiplicity of 10. At 6 h after infection, the cells were fixed, and immunofluorescence analysis was carried out with an anti-ICP0 antibody. VP22 is shown in green; ICP0 is shown in red. (C) Vero cells were infected with an HSV-1 recombinant expressing VP26-YFP at a multiplicity of 10. At 6 h after infection, the cells were fixed, and immunofluorescence analysis was carried out with an anti-ICP0 antibody. VP26 is shown in green; ICP0 is shown in red. Insets show magnified images of individual nuclear domains.