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CDK2 activity in E7-expressing, differentiated HFKs. CDK2 activity of HFKs was assessed after 1 (A) or 5 (B) days of growth in differentiation medium (DM). Histone H1 (HH1) was used as the substrate. Percentages of kinase activity (% activity) relative to the levels retained by matched control cells maintained in growth medium (GM) are indicated. The lower blots in each panel are lysate input controls probed with an anti-actin antibody (I19; Santa Cruz).
