TABLE 2.
Vector or protein transduced | % of cells with indicated peptide concn (μg/ml) ina:
|
|||||
---|---|---|---|---|---|---|
G1
|
S
|
G2/M
|
||||
0 | 10 | 0 | 10 | 0 | 10 | |
Empty vector | 54.1 ± 2.1 | 70.9 ± 1.2 | 22.9 ± 3.6 | 3.9 ± 1.3 | 20.8 ± 4.0 | 21.7 ± 2.5 |
E7 WT | 51.1 ± 4.4 | 64.0 ± 2.1 | 24.4 ± 2.7 | 10.1 ± 1.3 | 22.0 ± 3.0 | 21.3 ± 3.5 |
E7 H2P | 53.8 ± 4.1 | 76.6 ± 0.2 | 23.0 ± 0.7 | 5.2 ± 0.5 | 20.9 ± 1.5 | 16.7 ± 2.3 |
E7 Δ21-24 | 53.0 ± 2.9 | 64.3 ± 3.6 | 21.3 ± 3.2 | 8.8 ± 2.2 | 23.1 ± 1.3 | 21.4 ± 2.1 |
E7 E46A | 46.1 ± 2.5 | 67.9 ± 4.6 | 26.7 ± 1.9 | 9.6 ± 0.4 | 24.6 ± 0.9 | 20.3 ± 2.0 |
E7 CVQ68-70AAA | 48.3 ± 2.5 | 70.3 ± 5.9 | 25.1 ± 2.6 | 5.6 ± 0.8 | 24.2 ± 2.5 | 22.0 ± 5.1 |
E7 Δ79-83 | 54.1 ± 3.6 | 76.3 ± 1.0 | 24.3 ± 1.3 | 6.4 ± 2.7 | 19.5 ± 1.9 | 16.0 ± 0.3 |
Numbers are percentages of cells expressing vector or protein in the indicated phase of the cell cycle and with the indicated peptide concentration determined as described in Materials and Methods. The percentages are averages of values from three experiments ± standard deviations.