Figure 5.—

Levels of V-ATPase subunits in whole-cell lysates isolated from wild-type and mutant yeast strains. Whole-cell lysates were prepared from the indicated strains as described in materials and methods and an equal amount of lysate protein was loaded in each lane. Whole-cell lysates were separated by SDS-polyacrylamide gel electrophoresis, the separated proteins were transferred to nitrocellulose, and immunoblots were probed as described in Figure 4.