TABLE 1.

Trypsin digestion

Peptide	Retention time (min)	Sequencea	Measured mass (Da)	Theoretical mass (Da)	Measured reduced mass
T1	62.91	ENITQWNLSDNGTNGIQHAMYLR	2,820.3	2,674.3	—b
T2	43.31	SLBGIWPGK	994.0	993.55	—
T4	43.31	GVPTHLWTDVELK	1,378.5	1,378.75	—
T5b/T6	32.80	SEGTNYTCCKLQR	1,500.5f	1,499.67	1,502.5
T5/T6	48.85	EIQGMMDASEGTNYTCCKLQR	2,375.8f	2,375.02	2,377.8d
T7	17.95	HEWNK	712.5	712.34	—
T8 + T3	72.61	HGWCNWHNIDPWIQLMNRTCK	2,680.4f	2,679.27	2,320.2e
		ICK
T9	29	TQADLAEGPPVK	1,224.5	1,224.64	NTc
T11/T12	33.55	YDKDADINVVTQAR	1,606.6	1,606.8	NT

^aSeveral peptides were subjected to MS-MS. The elucidated sequence is underlined and bold.

^b—, no difference between oxidized and reduced fragments.

^cNT, not tested.

^dTheoretical mass of reduced T5/T6, 2,377.0 Da.

^eTheoretical mass of T8, 2,319.1 Da.

^fFragment is approximately 1 Da larger due to Asn to Asp modification during N deglycosylation.