TABLE 1.
Peptide | Retention time (min) | Sequencea | Measured mass (Da) | Theoretical mass (Da) | Measured reduced mass |
---|---|---|---|---|---|
T1 | 62.91 | ENITQWNLSDNGTNGIQHAMYLR | 2,820.3 | 2,674.3 | —b |
T2 | 43.31 | SLBGIWPGK | 994.0 | 993.55 | — |
T4 | 43.31 | GVPTHLWTDVELK | 1,378.5 | 1,378.75 | — |
T5b/T6 | 32.80 | SEGTNYTCCKLQR | 1,500.5f | 1,499.67 | 1,502.5 |
T5/T6 | 48.85 | EIQGMMDASEGTNYTCCKLQR | 2,375.8f | 2,375.02 | 2,377.8d |
T7 | 17.95 | HEWNK | 712.5 | 712.34 | — |
T8 + T3 | 72.61 | HGWCNWHNIDPWIQLMNRTCK | 2,680.4f | 2,679.27 | 2,320.2e |
ICK | |||||
T9 | 29 | TQADLAEGPPVK | 1,224.5 | 1,224.64 | NTc |
T11/T12 | 33.55 | YDKDADINVVTQAR | 1,606.6 | 1,606.8 | NT |
Several peptides were subjected to MS-MS. The elucidated sequence is underlined and bold.
—, no difference between oxidized and reduced fragments.
NT, not tested.
Theoretical mass of reduced T5/T6, 2,377.0 Da.
Theoretical mass of T8, 2,319.1 Da.
Fragment is approximately 1 Da larger due to Asn to Asp modification during N deglycosylation.