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. 2002 Oct;76(20):10195–10202. doi: 10.1128/JVI.76.20.10195-10202.2002

FIG. 2.

FIG. 2.

Northern blotting indicates that deletion variants do not accumulate pgRNA. Deletion of nt 2650 to 2671 or nt 686 to 717 results in accumulation of less pgRNA. Extending the nt 686-to-717 deletion to nt 840 restores accumulation of pgRNA. LMH cells were transfected with plasmids to express DHBV and GFP RNA, respectively. Poly(A)+ RNA was isolated from cells and analyzed by Northern blotting. Lane 1, wild-type RNA standard; lane 2, D686-717 RNA; lane 3, D2650-2671 RNA; lane 4, D686-840 RNA. Blots were hybridized with two probes, one that detects DHBV plus-strand sequence between nt 988 and 1665 and one that detects GFP mRNA. Positions of pregenomic class (PG), subgenomic class (SG), and GFP RNA are indicated on the left side.